ABSTRACT
We systematically reviewed the results of the studies on expression regulation, biological functions, and clinical prognostic significance of CASP8AP2 gene. At present, the studies showed that the expression of CASP8AP2 gene was regulated by Homeobox proteins and DNA methylation, and could be silenced by miRNA-210. This protein was involved in apoptosis mediated by FAS and TNFα, NF-κB activation mediated by TNFα, regulation of gene expression induced by glucocorticoid and mineralocorticoid receptor, comprising Cajal body and histone locus body, transcription of replication-dependent histone, 3' end processing of histone, regulation of S phase progression, in addition to functioning as coactivator of transcription factors c-Myb and p73 to activating many genes' expression. On the other hand, low expression of CASP8AP2 gene was associated with relapse in childhood ALL. The deletion of this gene was related to the poor prognosis of children with T-ALL and T lymphoblastic lymphoma. Furthermore, 3 SNPs in this gene were possibly correlated with genesis of diffuse large B cell lymphoma and childhood leukemia. In conclusions, CASP8AP2 was a multifunctional protein. It could function to regulate cell proliferation, apoptosis, and gene expression. In childhood hematological malignancies, CASP8AP2 was a promising molecular marker with prognostic significance. Some SNPs were possibly correlated with leukemo- and lymphomogenesis.
Subject(s)
Humans , Apoptosis , Apoptosis Regulatory Proteins , Calcium-Binding Proteins , DNA Methylation , Gene Expression , Gene Expression Regulation , Histones , NF-kappa B , Precursor T-Cell Lymphoblastic Leukemia-Lymphoma , Prognosis , RecurrenceABSTRACT
<p><b>OBJECTIVE</b>To observe the transcriptional expression of enamelin in developing postnatal rat first mandibular molar germs, for further studies of functions of enamelin in enamel development and mineralization.</p><p><b>METHODS</b>Tissue slices of first mandibular molar germ of rat 1, 3, 7, 10, 14 days after birth were prepared. The enamelin mRNA expression was identified by in situ hybridization.</p><p><b>RESULTS</b>Enamelin mRNA was observed in both ameloblast and odontoblast in 1-10 day old rat postnatal first mandibular molar germs. Enamelin mRNA appeared very weakly at 1st day, and increased through 3rd day, reached the maximum at 7th day, and reduced at 10th day and became negative at 14th day postnatally; while the expression of enamelin mRNA in odontoblast maintained lower from 1st to 10th day and negative at 14th day postnatally.</p><p><b>CONCLUSION</b>Enamelin gene transcriptional expression lasts from preameloblast to maturation ameloblast, which suggests that enamelin may participate in the development of enamel and mantle dentin.</p>
Subject(s)
Animals , Rats , Ameloblasts , Metabolism , Dental Enamel Proteins , Genetics , Gene Expression Regulation , In Situ Hybridization , Molar , Embryology , Odontoblasts , Metabolism , RNA, Messenger , Tooth Germ , Metabolism , Transcription, GeneticABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and biological effect of Shh during late bell stage by morphological and semi-quantitative analysis.</p><p><b>METHODS</b>Tooth germs were selected from new born Bal b/c mouse (P1, P2, P3, P5, P7). Semi-quality of Shh was measured by Western Blot and the expression place and strength of Shh were observed by in situ hybridization.</p><p><b>RESULTS</b>Shh was expressed in the ameloblast layer during late bell stage; the expression strength was high in secretive period and decreased with development; the active N-section was detectable before P3.</p><p><b>CONCLUSION</b>Shh expresses specially in the ameloblast layer in late bell stage, and expression quality is related to the function of ameloblasts.</p>